王 盼

姓名:王盼

职称:工学博士/硕士生导师

部门:生物工程系

电子邮箱:panwanghgxy@nwu.edu.cn

办公地点:太白校区科技楼五楼


主要研究领域

发酵工程、微生物酶学、合成生物学


工作及教育经历

2019.12~至今,437必赢会员中心在职博后;

2018.07~至今,437必赢会员中心,讲师;

2012.09~2018.06,华南理工大学,发酵工程,博士(直博);

2008.09~2012.07,华南理工大学,生物工程,本科


指导学生项目及竞赛

12020年陕西省大学生创新创业训练计划项目(S202010697500)—重组糖苷酶在毕赤酵母中的高效表达及催化人参皂苷的条件优化。指导学生:袁博韬,付健铭,李思齐;

22021年“国际基因工程机器大赛(International Genetically Engineered Machine competition,简称iGEM)”。指导学生:李佳程,武阳,常泽文,马宇欣,牛子阳等;获全球银奖;

32021年“生物+”国际青年科技作品展评大会—人参皂苷的转化之旅。指导学生:付健铭,李智杰,李佳程等;获优秀作品奖。


主要科研项目

1、国家自然科学基金青年项目(22108229),β-葡萄糖苷酶高效分泌及催化合成稀有人参皂苷Compound K的机制研究,2022.01-2024.12。在研,主持;

2、陕西省教育厅专项科研计划项目(20JK0938),微生物转化法高效制备稀有人参皂苷CK的研究,2020.01-2021.12。在研,主持;

3、西安市科协青年人才托举计划项目,基于蛋白质组学的黑曲霉耐受人参皂苷Rb1的胁迫响应机制研究,2019.11-2021.11。已结题,主持;

4、437必赢会员中心科研启动基金(338050059),高转化及耐受人参皂苷Rb1菌株的筛选及机制研究,2018.11-2020.11。已结题,主持;

5、国家重点研发计划项目-合成生物学(2009YFA0905200),人造蛋白质合成的细胞设计构建及应用,2020.01-2024.12。在研,骨干成员,参加;

6、国家自然科学基金面上项目(21978236),人参皂苷Rk3靶向PD-L1调节肠道菌群对肝癌的抑制及作用机制研究,2020.01-2023.12。在研,参加;

7、国家自然科学基金面上项目(21978235),人参皂苷Rg5通过调节肠道菌群缓解II型糖尿病代谢紊乱的作用机制研究,2020.01-2023.12。在研,参加。


研究成果

1. Xue Bai, Rongzhan Fu, ZhiguangDuan, Pan Wang, Chenhui Zhu, Daidi Fan. Ginsenoside Rk3 alleviates gut microbiotadysbiosis and colonic inflammation in antibiotic-treated mice. Food Research International, 2021, 146(6), 110465.

2.Jing Cao, Pan Wang, Yannan Liu,Chenhui Zhu, Daidi Fan. Double crosslinked HLC-CCS hydrogel tissue engineering scaffold for skin wound healing[J]. International Journal of Biological Macromolecules, 2020, 155:625-635.

3. Gang Liu, Zhiguang Duan, Pan Wang, Daidi Fan, Chenhui Zhu. Purification, characterization, and hypoglycemic properties from Eurotium cristatum spores in Fuzhuan brick tea. RSC Advances, 2020, 10(37):22234-22241.

4. Mimi Xing, Rongzhan Fu, Yannan Liu, Pan Wang, Pei Ma, Chenhui Zhu, Daidi Fan. Human-like collagen promotes the healing of acetic acid-induced gastric ulcers in rats by regulating NOS and growth factors[J].Food & function, 2020,11(5):4123-4137.

5.Pan Wang, Yin Lin, Chenjuan Zou, Fengguang Zhao, Shuangyan Han. Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia patoris[J]. BMC Microbiology, 2020, 20(262):1-12.

6. Chengjuan Zou, Pan Wang, Shuli Liang, Ying Lin. Deletion of Gcw13 represses autophagy in Pichia pastoris cells grown in methanol medium with sufficient amino acids[J].Biotechnology Letters, 2019,41(12):1423-1431.

7. Chengjuan Zou, Pan Wang, Shuli Liang, Shuangyan Han, Suiping Zheng, Ying Lin. Deletion of the GCW13 gene derepresses Gap1-dependent uptake of amino acids in Pichia pastoris grown on methanol as the sole carbon source[J]. Biochemical and Biophysical Research Communications, 2018, 501(1):226-231.

8. Pan Wang, Li Zhang, Rebecca Fisher, Meiqi Chen, Shuli Liang, Shuangyan Han, Suiping Zheng, Haixin Sui, Ying Lin. Accurate analysis of fusion expression of Pichia pastoris glycosylphosphatidyl- inositol-modified cell wall proteins[J].Journal of Industrial Microbiology & Biotechnology, 2017,44(9):1355-1365.

9. Kun Zhang, Zi Jin, Pan Wang, Suiping Zheng, Shuangyan Han, Ying Lin. Improving the catalytic characteristics of lipase-displaying yeast cell by hydrophobic modification[J]. Bioprocess and Biosystems Engineering, 2017, 40(11):1689-1699.

10. Jie He, Chyongere Hsieh, Yongping Wu, Thomas Schmelzer, Pan Wang, Ying Lin, Michael Marko, Haixin Sui. Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM[J]. Journal of Structural Biology, 2017,199(2):114-119.

11. Pan Wang, Jie He, Yufei Sun, Matthew Reynolds, Li Zhang, Shuangyan Han, Shuli Liang, Haixin Sui, Ying Lin. Display of fungal hydrophobin on the Pichia pastoris cell surface and its influence on Candida antarctica lipase B[J].Applied microbiology and biotechnology, 2016, 100(13):5883-5895.

12. 王盼, 朱晨辉, 赵婧, 范代娣. 合成生物学在蛋白质功能材料领域的研究进展[J]. 合成生物学,2021,2(1):46-58.

13. 赵婧, 王盼, 刘彦楠, 傅荣湛, 段志广, 范代娣.人参皂苷的定向生物转化研究进展[J]. 化工进展,2021, 40(3):1238-1247.